The influence of metal substitution over the catalytic constants for NagA might be used to evaluate the contributions created by these divalent cations on enzymatic exercise (22, 23). The connection on the value of k
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The lack of activity is not really resulting from a diminished capability to bind divalent daftar naga26 cations Because the metal information from the purified mutants was just like the wild type enzyme. The catalytic Houses of these mutants are totally in keeping with the proposed position of the residue during the chemical system as the key normal base for activation with the hydrolytic water molecule and the following protonation with the leaving team amine.
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